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Total Nucleic Acid Extraction - Pathogens

CellInsightTM Total Nucleic Acid Extraction Kit – Pathogens (Ref#CM9100) is suitable for use with blood samples, liquid samples (e.g. plasma, serum, urine, sputum, swab washes), tissue samples, faeces.

The CellInsightTM Total Nucleic Acid Extraction Kit – Pathogens (Ref#CM9100) is intended for manual and automated extraction of total nucleic acids (DNA and RNA) from a wide range of biological samples. Processing time for the preparation of 96 samples is about 40 minutes. The kit requires no phenol/chloroform extraction, no alcohol precipitation and eliminates the need for centrifugation, vacuum filtration or column separation. The obtained total nucleic acids can be used directly as template for downstream applications such as PCR, real-time PCR and real-time RT-PCR.

Samples are lysed under denaturing conditions by adding lysis buffer and (optional) Proteinase K. During the incubation the released total nucleic acids bind to magnetic beads. After magnetic separation and discard of supernatant, the beads are washed using alcohol containing wash buffers to remove contaminants and salts. Air drying removes alcohol traces from the wash steps. Purified Total Nucleic Acids are eluted with low-salt elution buffer or water. The obtained Total Nucleic Acids can be used for direct analysis by downstream applications such as PCR, real-time PCR and real-time RT-PCR or should be stored at < -20°C for later analysis.

CellInsightTM Total Nucleic Acid Extraction Kit – Pathogens (Ref#CM9100) is suitable for use with blood samples, liquid samples (e.g. plasma, serum, urine, swab washes), tissue samples, faeces. The kit provides reagents for extraction of total nucleic acids from 200 µL liquid samples or 200µL homogenized tissue samples, cells or suspended faeces. Total Nucleic Acids are finally eluted in a volume of 100 µL elution buffer or water.

The CellInsightTM Total Nucleic Acid Extraction Kit – Pathogens (Ref#CM9100) kit is compatible with KingFisherTM Flex Magnetic Particle Processor.

CellInsightTM Total Nucleic Acid Extraction Kit – Pathogens (Ref#CM9100) is intended for Research Use Only (RUO).

For further information on our Total Nucleic Acid Extraction Kit – Pathogens, please refer to the Documents Centre of this page.

 

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CM9100 Total Nucleic Acid Extraction - Pathogens for 96 reactions 0.00 €