GMO Testing - General Context
A genetically modified organism (GMO) is a living organism whose genetic heritage has been modified by the insertion of “an extra piece” of DNA under laboratory conditions. Using genetic engineering technologies, combinations of genes that cannot occur in nature or through classical crossbreeding methods, can be created. Insertion of “an extra piece” od DNA is based on circular cloning vectors (plasmids) containing several genetic elements. These circular cloning vectors, once integrated into the host genome, take a linear structure. Detection of the integrated “extra piece” of DNA is performed by real-time PCR, the absolute gold standard in GMO analysis.
CellMadeTM Laboratories established a GMO screening approach detecting GMOs, including third and fourth generation GMOs, on a broad range of products: seeds, raw materials and processed products. The analytical approach allows for the screening of following markers: p35S, tNOS, PAT, EPSPS, Cry1Ab/Ac, t35S, nptII, BAR, pFMV, pNOS, tE9. The generic screening markers are complemented with Soy-specific (currently: 8 GM Soy events) and corn-specific markers (currently: 2 GM Corn events).
In all cases, extracted DNA is quality controlled by 28S rRNA gene (universal eukaryotic marker) amplification. On special request the GMO screening approach can be complemented with taxa specific assays for soy, rice, potato, sugar beet, tomato, papaya, flax, rapeseed, cotton, corn and wheat.
CellMadeTM Laboratories’ excellent know-how in molecular biology make us the ideal partner to accompany you in your GMO monitoring plan with regard to the various European regulations.