Food Safety and Environmental Testing

Viral RNA extraction

The CellInsightTM Viral RNA Extraction Kit (REF#CM-9100) is intended to isolate and purify viral RNA for subsequent analysis by real-time RT-PCR. The CellInsightTM Viral RNA Extraction Kit (REF#CM-9100) is compatible with a broad range of biological samples, such as nasopharyngeal swap and salivary samples. The kit is designed for use with real-time RT-PCR applications. The CellInsightTM Viral RNA Extraction Kit (REF#CM-9100) provides reagents for 960 reactions for the isolation and purification of viral RNA from a broad range of biological samples, such as nasopharyngeal and salivary samples. The CellInsightTM Viral RNA Extraction Kit (REF#CM-9100) is automatable on a large variety of robotic platforms that can handle magnetic beads.

The principle of the CellInsightTM Viral RNA Extraction Kit (REF#CM-9100) is based on the reversible absorption of RNA onto magnetic beads under appropriate buffer conditions. Biological samples are lysed by incubation with lysis/binding buffer containing chaotropic ions. Binding of the viral RNA to the magnetic microbeads is ensured by the presence of isopropanol in the lysis/binding buffer. The magnetic beads are then washed with three wash buffers to remove all traces of salts and contaminants. Finally, ultrapure RNA is eluted with the provided elution buffer. Purified viral RNA can be further analyzed by real-time RT-PCR methods. The proprietary chemistry of the CellInsightTM Viral RNA Extraction Kit (REF#CM-9100) avoids the use of proteinase K and carrier RNA. The proprietary chemistry also avoids the air-drying step of the magnetic beads before elution. Washing step 3 removes all traces of isopropanol and other impurities and allows you to proceed directly to the elution step to obtain ultrapure RNA ready for highly sensitive applications by real-time RT-PCR.

The CellInsightTM Viral RNA Extraction Kit (REF#CM-9100) as such does not produce analytical results. The user should use and validate the CellInsightTM Viral RNA Extraction Kit (REF#CM-9100) in conjunction with a real-time RT-PCR method for the qualitative detection of e.g., SARS-CoV-2 RNA. It is the user's responsibility to use appropriate controls (internal controls, extraction controls, positive/negative controls). 

The CellInsightTM Viral RNA Extraction Kit (REF#CM-9100) is for research use only.