BiobankCell BiologyBio-Analysis

Epithelial Cells

Ethically sourced and fully traceable human primary disease epithelial cells. Functional and biochemical characterization. For studying metabolic disease: obesity, diabetes type II, NAFLD.

CellMade Human Disease Primary Liver Epithelial Cells and Pancreatic Epithelial Cells.

Product Description:

  • HLiEpC are isolated from normal human liver tissue.
  • HPaEpC are isolated from normal human pancreatic tissue.
  • Human tissues are sourced via CellMade’s own tissue acquisition network under standardized conditions (temperature, transport medium, transport, delay, …). CellMade manages its own biobank and guarantees full compliance and traceability according to the laws of France.

Isolation method

The method we use to isolate primary epithelial cells was developed based on a combination of established and proprietary methods. Cells are pre-coated with EpCAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.

Epithelial cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for t = 2 min and incubated in CellMade’s Complete Growth Medium, depending on the selected cell model, generally for 3-7 days. Cultures are then expanded. Cells at passage 3 are detached from cell culture flasks and immediately cryopreserved. Each vial contains at least 0.5x106 cells per mL.

Product Testing and Functional Characterization

  • HLiEpc and HPaEpC from CellMade display typical polygonal shape with regular dimensions under light microscopy.
  • Standard: HLiEpC and HPaEpC are tested for expression of epithelial cell-marker E-cadherin by immunofluorescence staining methods and cytometry.
  • Functionally characterized: HLiEpC and HPaEpC are tested for reactivity after cellular stimuli:  TGF-ß1 activation of Collagen (Col1A1) expression measured in time-response/dose-response manner via ELISA methods
  • All CellMade primary cell models test negative for TVO, Moulds, Yeasts and Mycoplasmas as tested by PCR methods;
  • All CellMade primary cell models test negative for HIV-1, HIV-2, Hepatitis-B and Hepatitis-C as tested by PCR methods and Antibody-methods;
  • All CellMade primary cell models are accompanied by a Certificate of Analysis.

Biochemical characterization

  • CellMade has established rigorous biochemical quality control programs ensuring the qualification of normal and disease primary cell models; 

For further information on our human primary epithelial cell products, please refer to the Documentation Centre of this page.


Products

Ref Products Associated Products Price
CM2200 Cryoconserved Disease Functionally Characterised Human Liver Epithelial Cells (HLiEpC) (>500,000 cells), adult Diabetes Type II (+) 886.00 €
CM2201 Cryoconserved Disease Functionally Characterised Human Liver Epithelial Cells (HLiEpC) (>500,000 cells), adult Diabetes Type II (+), NAFLD (+) 886.00 €
CM2202 Cryoconserved Disease Functionally Characterised Human Liver Epithelial Cells (HLiEpC) (>500,000 cells), adult NAFLD (+) 886.00 €
CM2203 Cryoconserved Disease Functionally Characterised Human Liver Epithelial Cells (HLiEpC) (>500,000 cells), adult Obesity (+) 886.00 €
CM2204 Cryoconserved Disease Functionally Characterised Human Liver Epithelial Cells (HLiEpC) (>500,000 cells), adult Obesity (+), Diabetes Type II (+) 886.00 €
CM2205 Cryoconserved Disease Functionally Characterised Human Liver Epithelial Cells (HLiEpC) (>500,000 cells), adult Obesity (+), Diabetes Type II (+), NAFLD (+) 886.00 €
CM2206 Cryoconserved Disease Functionally Characterised Human Liver Epithelial Cells (HLiEpC) (>500,000 cells), adult Obesity (+), NAFLD (+) 886.00 €
CM2207 Cryoconserved Disease Functionally Characterised Pancreatic Epithelial Cells (HPaEpC) (>500,000 cells), adult Diabetes Type II (+) 886.00 €
CM2208 Cryoconserved Disease Functionally Characterised Pancreatic Epithelial Cells (HPaEpC) (>500,000 cells), adult Diabetes Type II (+), NAFLD (+) 886.00 €
CM2209 Cryoconserved Disease Functionally Characterised Pancreatic Epithelial Cells (HPaEpC) (>500,000 cells), adult NAFLD (+) 886.00 €
CM2210 Cryoconserved Disease Functionally Characterised Pancreatic Epithelial Cells (HPaEpC) (>500,000 cells), adult Obesity (+) 886.00 €
CM2211 Cryoconserved Disease Functionally Characterised Pancreatic Epithelial Cells (HPaEpC) (>500,000 cells), adult Obesity (+), Diabetes Type II (+) 886.00 €
CM2212 Cryoconserved Disease Functionally Characterised Pancreatic Epithelial Cells (HPaEpC) (>500,000 cells), adult Obesity (+), Diabetes Type II (+), NAFLD (+) 886.00 €
CM2213 Cryoconserved Disease Functionally Characterised Pancreatic Epithelial Cells (HPaEpC) (>500,000 cells), adult Obesity (+), NAFLD (+) 886.00 €