Human Primary Cells - General Context
Human primary cells are cells that are isolated directly from human tissues or organs, and have not been immortalized or transformed. They are called "primary" because they are the first cells that are obtained from a tissue or organ, without any genetic modifications or alterations. Human primary cells are usually obtained from discarded or excess tissues during surgeries or biopsies, and can be cultured in vitro for a limited period of time. These cells retain the characteristics and functions of the tissue or organ from which they were derived, making them valuable tools for toxicology studies.
CellMade human functionally characterized primary cells are human primary cells that have been thoroughly tested and validated to ensure that they retain their original functions and properties in vitro. CellMade human functionally characterized primary cells must meet certain criteria, including:
- Retention of tissue-specific functions: The cells maintain functions and properties of the tissue or organ from which they were derived, such as the ability to produce specific enzymes, hormones, or other biomolecules.
- Validation of cell purity: The cells are thoroughly tested to ensure that they are pure and free from contamination by other cell types or microorganisms.
- Consistency in growth and behavior: The cells are consistent in their growth and behavior, with reproducible results across different experiments.
- Validation of biomarkers: The cells are validated using biomarkers, such as gene expression patterns, protein levels, or cell surface markers
Human functionally characterized primary cells, such as monocytes, hepatocytes, keratinocytes, and adipocytes, are valuable tools for predictive toxicology. The use of human functionally characterized primary cells in 2D and 3D culture formats in predictive toxicology has several advantages. In 2D culture, cells are grown on a flat surface, and this format is suitable for high-throughput screening and rapid toxicity assessments. In contrast, 3D culture allows cells to interact with each other and their surroundings, providing a more physiologically relevant environment that mimics the in vivo microenvironment.
Advantages of using CellMade human functionally characterized primary cells in predictive toxicology include:
- Improved accuracy: CellMade human functionally characterized primary cells are more representative of the human physiology than cell lines derived from animal sources or immortalized cell lines, providing more accurate and relevant data.
- Better predictivity: 2D and 3D cultures of CellMade human functionally characterized primary cells can better predict in vivo responses and provide a more comprehensive understanding of the mechanisms underlying toxicity.
- More relevant data: CellMade human functionally characterized primary cells cultured in 2D and 3D formats provide more physiologically relevant data, enabling better identification and mitigation of toxicological risks.
- More consistent results: CellMade human functionally characterized primary cells can provide more consistent and reproducible results than traditional cell lines, reducing the need for repeat experiments and saving time and resources.
In summary, the use of CellMade human functionally characterized primary cells in both 2D and 3D culture formats in predictive toxicology offers significant advantages over traditional cell lines, providing more accurate, relevant, and predictive data for identifying and mitigating toxicological risks