Functionally characterized CD14+ Monocytes

Maximize the sensitivity and reproducibility of your pyrogen and cytokine release syndrome (CRS) assays with our premium, high-purity cryopreserved human CD14+ Monocytes. Sourced from fully consented, healthy donors under strict IRB-approved protocols, our purified monocytes provide a superior, ethically sound solution for your most critical immuno-safety and lot-release testing.

The Scientific Advantage: Why Use CD14+ Monocytes over PBMCs?

While PBMCs are a common source, CD14+ monocytes are the primary cell population responsible for pyrogen detection and the subsequent innate cytokine cascade. By using a purified population, you eliminate the significant donor-to-donor variability in monocyte percentages (which can range from 10-30%) and the confounding influence of other cell types (like lymphocytes) present in a mixed PBMC population. This use of a defined, pure population of the key responding cell dramatically increases assay sensitivity, specificity, and reproducibility, leading to a cleaner, more robust signal-to-noise ratio. This precision is essential for the accuracy of the Monocyte Activation Test (MAT) and for developing reliable in-vitro CRS models.

Each vial is guaranteed to contain a minimum of 5 million viable, high-purity (>90% CD14+) cells, cryopreserved using an optimized process to ensure high post-thaw viability and immediate functional performance.

Product Formats for Your Assay Needs

We offer flexible formats designed for the specific demands of pyrogen and CRS testing:

• Single Donor Vials: Ideal for assessing donor-to-donor variability in monocyte response, a critical factor in understanding population differences in pyrogen sensitivity and CRS risk.
• 4-Donor Pooled Vials: Provides a standardized, representative monocyte population by averaging individual donor responses. This format is the industry standard for robust, reproducible lot-release testing, such as the Monocyte Activation Test (MAT).

Guaranteed Functional Characterization

Move beyond basic cell counts and viability. Our monocyte lots are rigorously tested for the functional performance required by a pyrogen or CRS assay, providing you with the assurance that your cells are ready for immediate use.

Characterization data includes:

• TLR-Receptor Activation: Our monocytes are confirmed to be highly responsive to key TLR agonists (e.g., LPS for TLR4, R848 for TLR7/8), demonstrating their suitability for detecting a wide range of pyrogenic contaminants.
• Cytokine Release Profile: Each lot is tested for a strong, low-background cytokine response. We provide specific data on key pyrogen and CRS markers, including $IL-6$ and $TNF-\alpha$, ensuring a high signal-to-noise ratio and reliable assay performance.

All cells ship with a comprehensive Certificate of Analysis detailing viability, cell count, purity, serology testing, and lot-specific functional data. Ensure the consistency and reliability of your immuno-safety assays with our validated CD14+ Monocytes.