Cytokine ELISA kits

Explore our CRT and MAT-optimized CellMade™ Cytokine Detection Solutions, specifically engineered for the high-sensitivity quantitative determination of key human inflammatory cytokines in cell culture supernatants.

Comparative Kit Performance

The following table summarizes the detection specifications for our current specialized lineup:

Gene Knockout (KO) Validation: The Gold Standard

CellMade™ Cytokine Detection Solutions are validated using Gene Knockout (KO) technology to ensure maximum specificity. Gene Knockout is a genetic engineering technique where a specific gene is rendered non-functional, preventing the production of its corresponding protein.

In our validation process:

• Specific Recognition: The kits are tested against "KO cell lines" where the target cytokine gene has been disrupted.
• Proof of Accuracy: Truly specific kits show ND (Not Detected) in the knockout sample, even when stimulated under conditions that normally trigger protein expression.
• No Cross-Reaction: This rigorous testing confirms the antibodies specifically recognize the target protein and have no obvious cross-reaction with other analogues.

Core Product Features

• Sandwich ELISA Technology: Employs a reliable sandwich principle with pre-coated plates and biotin-conjugated detection antibodies.
• Rapid Workflow: Total detection duration is approximately 4 hours, excluding sample preparation and balancing.
• High Precision: Kits are subject to strict Quality Control, ensuring an Intra-assay and Inter-assay CV% typically below 10%.

Kit Components

CellMade™ Cytokine Detection Solutions kits contains all necessary reagents, including a dismountable microplate, lyophilized standards, 100X Biotin-labeled antibody, 100X HRP-Streptavidin conjugate (SABC), TMB substrate, stop solution, and dedicated dilution buffers.

Storage & Long-Term Stability

The CellMade™ Advantage

When you choose our CRT and MAT-optimized CellMade™ Cytokine Detection Solutions, you gain more than cells in a vial; you gain a comprehensive analytical partnership.

• Expert Technical Support: Every solution is backed by direct access to validation reports, detailed kit inserts, and expert consultation to help you optimize your specific workflow.

Procurement & Implementation

Access to CellMade™ Pharmaceutical Quality Control products is restricted to ensure peak performance and regulatory alignment. These products are available exclusively under a purchase license following a mandatory technical onboarding process:

• Feasibility & Method Development: Initial assessment and protocol design tailored to your specific application.

• Validation & Transfer: Product-specific validation followed by a formal method transfer to your facility.

• Implementation: Comprehensive on-site training to ensure seamless integration into your QC workflow.