Chemically Defined Media

CellInsight™ CD-Prime is a proprietary, serum-free, and chemically defined medium. It is meticulously formulated to support the robust culture, viability, and functional integrity of human Peripheral Blood Mononuclear Cells (hPBMCs) and purified CD14+ monocytes.

Engineered as a complete replacement for media supplemented with Fetal Bovine Serum (FBS) or other undefined components, this formulation provides a precisely controlled and optimized environment. It is the gold standard for sensitive immunological assays where accuracy, reproducibility, and regulatory compliance are non-negotiable.

Key Features & Advantages

This medium is designed to eliminate the fundamental problems associated with serum-based culture systems.

• 100% Chemically Defined & Serum-Free

Every component has a known chemical structure and precise concentration. This formulation is entirely free of animal-derived proteins, serum, and undefined hydrolysates, providing a clean and controlled system.

• Ultimate Reproducibility

By eliminating the batch-to-batch variability inherent in serum, CellInsight™ CD-Prime ensures exceptional consistency in cell performance. This is critical for standardizing protocols and ensuring that results are comparable over time and across different testing sites.

• Assured Pyrogen-Free Baseline

Formulated using only highly purified, qualified raw materials, this medium provides the highest assurance of being pyrogen-free. It mitigates the significant risk of contamination from endotoxins or adventitious agents (viruses, mycoplasma) often present in serum, thereby preventing false-positive results.

• Enhanced Assay Sensitivity

This medium provides a clean baseline free from assay interference. Unlike serum, it contains no endogenous cytokines, growth factors, or unknown factors that can create high background signals or mask the true cellular response to a stimulus. This dramatically increases the assay's signal-to-noise ratio and overall sensitivity.

Primary Applications

CellInsight™ CD-Prime is optimized for the most demanding applications involving primary human monocytes and PBMCs.

• Monocyte Activation Test (MAT): The ideal solution for pyrogen testing, compliant with pharmacopeial guidelines (e.g., Ph. Eur. 2.6.30). Its pyrogen-free and defined nature ensures a reliable negative control and accurate detection of contaminants.
• Cytokine Release Assays: Provides a true baseline for measuring cytokine secretion (e.g., IL-6, IL-1β, TNF-α) in response to stimuli.
• Immunological Research: Supports functional studies of monocyte and PBMC activation, differentiation (e.g., to macrophages or dendritic cells), and cell signaling.
• Cell-Based Assay Development: Delivers the stability and consistency required for developing and validating robust, high-throughput screening assays.

Quality & Specifications