Food Safety and Environmental Testing

COVID-19

Severe acute respiratory syndrome 2 (SARS-CoV-2) is the novel beta coronavirus that causes the respiratory disease COVID-19. The respiratory disease COVID-19 is transmitted to humans, infected within populations, via respiratory droplets that are spread during conversations, coughs and sneezes. Symptoms of COVID-19 range from mild illness (dry cough, fatigue, fever and shortness of breath) to severe illness and in some cases death. The elderly, immunocompromised, and those with cardiovascular disease, diabetes, and chronic respiratory disease were found to be at higher risk for severe disease. To date, patient screening and social distancing measures have proven to be the only ways to reduce transmission rates. The current pandemic of SARS-CoV2 infection has prompted the need to rapidly implement diagnostic tests for the detection of SARS-CoV2 RNA using real-time RT-PCR assays.

The CellInsightTM SARS-Cov-2 Detection Kit (REF#CM-9001) provides reagents for 96 detection reactions of SARS-CoV-2 RNA extracted and purified using CellInsightTM Viral RNA Extraction kit (REF#CM-9100) from patient nasopharyngeal and saliva specimens. The CellInsightTM SARS-Cov-2 detection kit (REF#CM-9001) is compatible with nasopharyngeal and salivary samples collected in “D-PBS” type transport buffers, typically indicated for downstream molecular biology based analysis.

The CellInsightTM Direct SARS-CoV-2 Detection Kit (REF#CM-9002) provides reagents for 96 reactions to release nucleic acids and detect SARS-CoV-2 RNA from patient nasopharyngeal specimens. The originality of the CellInsightTM Direct SARS-CoV-2 Detection Kit (REF#CM-9002) is that it includes a lysis buffer which allows nucleic acids to be released according to a very simple protocol: V = 4 μL of lysis buffer and V=4 μL of nasopharyngeal sample followed by an incubation of t=10 minutes at room temperature; then, V=8 μL of the mixture is added to V=12 μL of rRT-PCR mix. The CellInsightTM Direct SARS-CoV-2 detection kit (REF#CM-9002) is compatible with nasopharyngeal samples collected by swab and deposited in “D-PBS” type transport buffers, typically indicated for downstream molecular biology based analysis.

The CellInsightTM SARS-Cov-2 Detection Kit (REF#CM-9001) and the CellInsightTM Direct SARS-CoV-2 Detection Kit (REF#CM-9002) are both CE-IVD labelled. Both kits are listed on the French national list of authorized COVID-19 PCR testing reagents (French Ministry of Health) consultable at https://covid-19.sante.gouv.fr/tests