BiobankCell BiologyBio-Analysis

Fibroblasts

Ethically sourced and fully traceable human primary fibroblasts. Functional and biochemical characterization for more meaningfull results.

CellMade Human Primary Cardiac Fibroblasts (HCF), Aorta Fibroblasts (HAoF), Dermal Fibroblasts (HDF), Liver Fibroblasts (HLiF) and Pancreatic Fibroblasts (HPaF)

Product Description

  • HCF are isolated from normal human heart tissue.
  • HAoF are isolated from normal human aorta.
  • HDF are isolated from normal human skin tissue (abdominal, breast or facial)
  • HLiF are isolated from normal human liver tissue.
  • HPaF are isolated from normal human pancreatic tissue.
  • Human tissues are sourced via CellMade’s own tissue acquisition network under standardized conditions (temperature, transport medium, transport, delay, …). CellMade manages its own biobank and guarantees full compliance and traceability according to the laws of France.

Isolation method

The method we use to isolate primary fibroblasts was developed based on a combination of established and proprietary methods.  Fibroblasts are pre-coated with fibroblast-specific antigen antibody, and selected with magnetic beads pre-coated with secondary antibody.

Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for t = 2 min and incubated in CellMade’s  Complete Growth Medium, depending on the selected primary cell model, generally for 3-7 days. Cultures are then expanded. Cells at passage 1 are detached from cell culture flasks and immediately cryopreserved. Each vial contains at least 0.5x106 cells per mL.

Cells can be expanded for up to five (5) passages under the cell culture conditions specified by CellMade.

Product Testing and Functional Characterization

  • HCF, HAoF, HDF, HLiF and HPaF from CellMade display typical fusiform appearance under light microscopy.
  • Standard: HCF, HAoF, HDF, HLiF and HPaF are tested negative for the expression of the von Willebrand Factor VIII, cytokeratin 18, and α-smooth muscle actin by immunofluorescence staining methods and cytometry.
  • Functionally characterized: Transforming growth factor-ß (TGF ß) stimulation of total protein and collagen synthesis, measured in time-response/dose -response manner via total protein measurement and ELISA.
  • All CellMade primary cell models test negative for TVO, Moulds, Yeasts and Mycoplasmas as tested by PCR methods.
  • All CellMade primary cell models test negative for HIV-1, HIV-2, Hepatitis-B and Hepatitis-C as tested by PCR methods and Antibody-methods.
  • All CellMade primary cell models are accompanied by a Certificate of Analysis.

Biochemical characterization

  • CellMade has established rigorous biochemical quality control programs ensuring the qualification of normal and disease primary cell models.

For further information on our human fibroblasts, please refer to the Documentation Centre of this page.

Contact us

CellMade Laboratoire

20, impasse des Sources
73310 RUFFIEUX

T : 04 57 34 53 02


Products

Ref Products Associated Products Price
CM1300 Cryoconserved Cardiac Fibroblasts (HCF) (>500,000 cells), adult Complete Growth Medium CM5300 339.00 €
CM1305 Cryoconserved Functionally Characterised Cardiac Fibroblasts (HCF) (>500,000 cells), adult Complete Growth Medium CM5300 443.00 €
CM1301 Cryoconserved Aorta Fibroblasts (HAoF) (>500,000 cells), adult Complete Growth Medium CM5301 339.00 €
CM1306 Cryoconserved Functionally Characterised Aorta Fibroblasts (HAoF) (>500,000 cells), adult Complete Growth Medium CM5301 443.00 €
CM1302 Cryoconserved Dermal Fibroblasts (HDF) (>500,000 cells), adult Complete Growth Medium CM5302 264.00 €
CM1307 Cryoconserved Functionally Characterised Dermal Fibroblasts (HDF) (>500,000 cells), adult Complete Growth Medium CM5302 345.00 €
CM1303 Cryoconserved Liver Fibroblasts (HLiF) (>500,000 cells), adult Complete Growth Medium CM5303 339.00 €
CM1308 Cryoconserved Functionally Characterised Liver Fibroblasts (HLiF) (>500,000 cells), adult Complete Growth Medium CM5303 443.00 €
CM1304 Cryoconserved Pancreatic Fibroblasts (HPaF) (>500,000 cells), adult Complete Growth Medium CM5304 339.00 €
CM1309 Cryoconserved Functionally Characterised Pancreatic Fibroblasts (HPaF) (>500,000 cells), adult Complete Growth Medium CM5304 443.00 €
CM1310 Proliferating Cardiac Fibroblasts (HCF) (2x T75 flasks), adult Complete Growth Medium CM5300 589.00 €
CM1315 Proliferating Functionally Characterised Cardiac Fibroblasts (HCF) (2x T75 flasks), adult Complete Growth Medium CM5300 693.00 €
CM1311 Proliferating Aorta Fibroblasts (HAoF) (2x T75 flasks), adult Complete Growth Medium CM5301 589.00 €
CM1316 Proliferating Functionally Characterised Aorta Fibroblasts (HAoF) (2x T75 flasks), adult Complete Growth Medium CM5301 693.00 €
CM1312 Proliferating Dermal Fibroblasts (HDF) (2* T75 flasks), adult Complete Growth Medium CM5302 459.00 €
CM1317 Proliferating Functionally Characterised Dermal Fibroblasts (HDF) (>500,000 cells), adult Complete Growth Medium CM5302 540.00 €
CM1313 Proliferating Liver Fibroblasts (HLiF) (2x T75 flasks), adult Complete Growth Medium CM5303 589.00 €
CM1318 Proliferating Functionally Charaterised Liver Fibroblasts (HLiF) (2x T75 flasks), adult Complete Growth Medium CM5303 693.00 €
CM1314 Proliferating Pancreatic Fibroblasts (HPaF) (2x T75 flasks), adult Complete Growth Medium CM5304 589.00 €
CM1319 Proliferating Functionally Characterised Pancreatic Fibroblasts (HPaF) (2x T75 flasks), adult Complete Growth Medium CM5304 693.00 €