BiobankCell BiologyBio-Analysis

Epithelial Cells

Ethically sourced and fully traceable human primary epithelial cells. Functional and biochemical characterization for more meaningfull results.

CellMade Human Primary Dermal Epidermal Keratinocytes, Liver Epithelial Cells, Mammary Epithelial Cells and Pancreatic Epithelial Cells.

Product Description:

  • HEK are isolated from normal human skin tissue.
  • HLiEpC are isolated from normal human liver tissue.
  • HEM are isolated from normal human breast tissue.
  • HPaEpC are isolated from normal human pancreatic tissue.
  • Human tissues are sourced via CellMade’s own tissue acquisition network under standardized conditions (temperature, transport medium, transport, delay, …). CellMade manages its own biobank and guarantees full compliance and traceability according to the laws of France.

Isolation method

The method we use to isolate primary epithelial cells was developed based on a combination of established and proprietary methods. Cells are pre-coated with EpCAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.

Epithelial cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for t = 2 min and incubated in CellMade’s Complete Growth Medium, depending on the selected cell model, generally for 3-7 days. Cultures are then expanded. Cells at passage 3 are detached from cell culture flasks and immediately cryopreserved. Each vial contains at least 0.5x106 cells per mL.

Product Testing and Functional Characterization

  • HEK, HLiEpc, HEM, HPaEpC from CellMade display typical polygonal shape with regular dimensions under light microscopy.
  • Standard: HEK, HLiEpC, HEM and HPaEpC are tested for expression of epithelial cell-marker E-cadherin by immunofluorescence staining methods and cytometry.
  • Functionally characterized: HEK, HLiEpC, HEM and HPaEpC are tested for reactivity after cellular stimuli:  TGF-ß1 activation of Collagen (Col1A1) expression measured in time-response/dose-response manner via ELISA methods
  • All CellMade primary cell models test negative for TVO, Moulds, Yeasts and Mycoplasmas as tested by PCR methods;
  • All CellMade primary cell models test negative for HIV-1, HIV-2, Hepatitis-B and Hepatitis-C as tested by PCR methods and Antibody-methods;
  • All CellMade primary cell models are accompanied by a Certificate of Analysis.

Biochemical characterization

  • CellMade has established rigorous biochemical quality control programs ensuring the qualification of normal and disease primary cell models; 

For further information on our human primary epithelial cell products, please refer to the Documentation Centre of this page.

 

Contact us

CellMade Laboratoire

20, impasse des Sources
73310 RUFFIEUX

T : 04 57 34 53 02


Products

Ref Products Associated Products Price
CM1200 Cryoconserved Epidermal Keratinocytes (HEK) (>500,000 cells), adult Complete Growth Medium CM5200 339.00 €
CM1204 Cryoconserved Functionally Characterised Epidermal Keratinocytes (HEK) (>500,000 cells), adult Complete Growth Medium CM5200 443.00 €
CM1201 Cryoconserved Liver Epithelial Cells (HLiEpC) (>500,000 cells), adult Complete Growth Medium CM5201 339.00 €
CM1205 Cryoconserved Functionally Characterised Liver Epithelial Cells (HLiEpC) (>500,000 cells), adult Complete Growth Medium CM5201 443.00 €
CM1202 Cryoconserved Mammary Epithelial Cells (HMEpC) (>500,000 cells), adult Complete Growth Medium CM5202 339.00 €
CM1206 Cryoconserved Functionally Characterised Mammary Epithelial Cells (HMEpC) (>500,000 cells), adult Complete Growth Medium CM5202 443.00 €
CM1203 Cryoconserved Pancreatic Epithelial Cells (HPaEpC) (>500,000 cells), adult Complete Growth Medium CM5203 339.00 €
CM1207 Cryoconserved Functionally Characterised Pancreatic Epithelial Cells (HPaEpC) (>500,000 cells), adult Complete Growth Medium CM5203 443.00 €
CM1208 Proliferating Human Epidermal Keratinocytes (HEK) (2x T75 flasks), adult Complete Growth Medium CM5200 589.00 €
CM1212 Proliferating Functionally Characterised Epidermal Keratinocytes (HEK) (2x T75 flasks), adult Complete Growth Medium CM5200 693.00 €
CM1209 Proliferating Liver Epithelial Cells (HLiEpC) (2x T75 flasks), adult Complete Growth Medium CM5201 589.00 €
CM1213 Proliferating Functionally Characterised Liver Epithelial Cells (HLiEpC) (2x T75 flasks), adult Complete Growth Medium CM5201 693.00 €
CM1210 Proliferating Mammary Epithelial Cells (HMEpC) (2x T75 flasks), adult Complete Growth Medium CM5202 589.00 €
CM1214 Proliferating Functionally Characterised Mammary Epithelial Cells (HMEpC) (2x T75 flasks), adult Complete Growth Medium CM5202 693.00 €
CM1211 Proliferating Pancreatic Epithelial Cells (HPaEpC) (2x T75 flasks), adult Complete Growth Medium CM5203 589.00 €
CM1215 Proliferating Functionally Characterised Pancreatic Epithelial Cells (HPaEpC) (2x T75 flasks), adult Complete Growth Medium CM5203 693.00 €