Mycotoxins - Purification columns

The analytical determination of trace-level contaminants in food and agricultural commodities is fundamentally challenged by the complexity of sample matrices. During the analysis of mycotoxins and pesticide residues, the presence of lipids, proteins, pigments, and carbohydrates creates significant "chemical noise" that can obscure the signal of target analytes and lead to inaccurate quantification.

CellMade Laboratories provides a comprehensive portfolio of purification columns and sample preparation tools—engineered specifically to bridge the gap between crude extraction and high-sensitivity detection. For specialized mycotoxin analysis, our solutions are categorized into three core technological platforms:

1. Immunoaffinity Columns (IAC) – Biological specificity.
2. Multifunction Cleanup Columns (MFC) – Rapid, high-throughput pass-through.
3. Molecularly Imprinted Polymer (MIP) Columns – Synthetic molecular recognition.

Each platform is designed to ensure strict laboratory compliance with international standards, including European Commission Regulation (EC) 1881/2006 and AOAC International guidelines.

Mycotoxin Analysis: Biological & Synthetic Platforms

Mycotoxins contaminate a vast array of crops, from peanuts and cereals to complex animal feeds. CellMade offers three distinct technologies to isolate these secondary metabolites with precision.

1. PriboFast® Immunoaffinity Columns (IAC)

PriboFast® IACs represent the gold standard for specificity.

• Mechanism: Utilizing highly specific monoclonal antibodies immobilized on a gel matrix, these columns target and capture only the intended mycotoxin.
• Operation: As the sample extract passes through, the target toxins bind to the antibodies while impurities are washed away. A subsequent elution step with a solvent (typically methanol) releases the purified, concentrated toxin.
• Portfolio: We offer high-recovery columns for single toxins (Aflatoxins, OTA, ZEN, DON) and Multi-Toxin Columns capable of purifying up to six toxin groups simultaneously.

2. PriboFast® Multifunction Cleanup Columns (MFC)

Designed for rapid screening and high-volume workflows, MFC columns utilize a "pass-through" principle rather than traditional bind-and-elute.

• Mechanism: A proprietary blend of composite fillers absorbs matrix impurities (fats, pigments, organic acids) while the analytes of interest pass through unimpeded.
• Efficiency: Cleanup is achieved in less than 30 seconds. These columns are exceptionally stable, featuring a shelf life of over 30 months at room temperature.
• Popular Models: M2200 (DON/NIV) and M1000 (multi-toxin) series.

3. PriboMIP™ Molecularly Imprinted Polymer Columns (MIP)

PriboMIP™ columns utilize "artificial receptors"—synthetic polymers engineered to mimic natural antibodies.

• Unmatched Stability: Unlike biological antibodies, MIPs are highly resistant to organic solvents, extreme pH levels, and high temperatures.
• Targeted Resolution: These are ideal for eliminating specific interferences. For example, the MIP-080-3 for Patulin effectively removes 5-hydroxymethylfurfural (5-HMF), a common interferent in apple-based matrices.

Product Selection & Strategic Matrix

Laboratory Automation and Quality Control

To minimize manual error and maximize throughput, CellMade Laboratories offers the Automatic Immunoaffinity Column Purification System. This system automates the loading, washing, and elution phases of the IAC workflow, ensuring standardized flow rates and superior recovery precision across every batch.

The CellMade Laboratories Advantage:

• Standards: For peak accuracy, we recommend the use of stable isotope-labeled internal standards. CellMade provides these standards to correct for matrix effects and ensure regulatory-grade data.
• Robust Reproducibility: Our columns demonstrate exceptionally low inter-assay and intra-assay coefficients of variation (CV).
• Expert Support: Every solution is backed by direct access to validation reports, detailed kit inserts, and expert technical consultation.