Food allergens - PCR kits

While protein-based detection is the industry standard, aggressive food processing—such as ultra-high temperature (UHT) treatment, autoclaving, or fermentation—can denature proteins, rendering them undetectable by traditional immunoassays.

CellMade Laboratories provides advanced Real-Time PCR (qPCR) solutions for the qualitative molecular detection of allergens. By targeting species-specific DNA sequences, qPCR provides a thermally stable diagnostic alternative for complex matrices.

Why Choose Molecular Detection?

Molecular verification serves as a critical secondary pillar in a defensible allergen management program, particularly for resolving cases of cross-reactivity and verifying highly processed ingredients.

• Thermal Stability: Genomic DNA remains detectable even after extensive thermal degradation of the protein matrix (e.g., in baked goods or retorted sauces).
• Taxonomic Precision: Essential for distinguishing between taxonomically related species, such as differentiating Celery (a regulated EU allergen) from parsley or carrots.
• Matrix Resilience: qPCR is less susceptible to "masking" effects common in fermented or highly acidic food products.

CellMade® Real-Time PCR Kits

Our qPCR analytical suite is engineered for high specificity and seamless integration into quality control workflows. These kits provide definitive qualitative "presence/absence" results to support Regulation (EU) No 1169/2011 compliance.

Technical Performance & Features

• High Sensitivity: Optimized to detect minute DNA traces, ensuring a robust safety margin.
• Multiplex Capabilities: Selected kits allow for the simultaneous detection of multiple allergens in a single run, increasing laboratory throughput.
• Universal Cycling Protocols: Streamlined parameters that are compatible with most standard laboratory Real-Time PCR instruments.
• Internal Control Systems: Each kit includes internal amplification controls (IAC) to prevent false-negative results caused by PCR inhibitors.
• External Control Systems: Each kit includes external extraction and amplification controls (EAC) to prevent false-negative results caused by method failures.

Streamlined Molecular Workflow

We offer a complete ecosystem for molecular allergen analysis, from efficient DNA extraction modules to validated amplification kits.

• Fast Turnaround: Go from sample to result in less then 2 hours.
• Clear Interpretation: Simple "Positive/Negative" calling based on cycle threshold (Ct) values.
• Expert Guidance: Our technical team provides support for master mix preparation, cycling optimization, and result validation.