BiobankCell BiologyBio-Analysis

Muscle Cells Media

Cost-effective and fully described media for human primary smooth muscle cells

CellMade muscle cell media are using basal medium and functionally tested growth supplements for optimized growth of HAoSMC and HUVSMC. 

The basal media are MCDB-131 based. MCDB-131 medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HMVEC). MCDB-131 medium is also used for other cell types, including human hepatocytes and smooth muscle cells.

MCDB-131 medium contains many components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins. These additions allow the medium to be supplemented with very low levels of serum or defined components. MCDB-131 medium contains no proteins or growth factors, and has to be supplemented.

MCDB 131 Medium uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5 to 10% (V/V) CO2 environment to maintain physiological pH.

  • CM3400 contains Phenol Red
  • CM3401 does not contain Phenol Red
  • CM3400 and CM3401 do not contain HEPES
  • CM3400 and CM3401 do contain L-Glutamine

The complete formulation and description of the basal media and the different growth supplements are available under the Documentation Centre of this page.


Products

Ref Products Associated Products Price
CM3400 Human Muscle Cells Basal Medium, W/ Phenol Red, W/ L-Glutamine, W/o HEPES, NAHCO3 buffered, 500 mL Complement with Cell Growth Supplements to obtain Growth Medium 58.00 €
CM3401 Human Muscle Cells Basal Medium, W/o Phenol Red, W/ L-Glutamine, W/o HEPES, NAHCO3 buffered, 500 mL Complement with Cell Growth Supplements to obtain Growth Medium 58.00 €
CM5400 Human Muscle Cells Growth Medium W/Phenol Red, W/L-Glutamine, W/o HEPES, NAHCO3 buffered and CM4401 growth supplements, for HUVSMC, 500 mL Complete Kit 121.00 €
CM5401 Human Muscle Cells Growth Medium, W/o Phenol Red, W/ L-Glutamine, W/ HEPES, NAHCO3 buffered and CM4401 growth supplements, for HUVSMC, 500 mL Complete Kit 121.00 €
CM5402 Human Muscle Cells Growth Medium W/Phenol Red, W/L-Glutamine, W/o HEPES, NAHCO3 buffered and CM4400 growth supplements, for HAoSMC, 500 mL Complete Kit 121.00 €
CM5101 Human Muscle Cells Growth Medium W/Phenol Red, W/L-Glutamine, W/o HEPES, NAHCO3 buffered and CM4400 growth supplements, for HAoSMC, 500 mL Complete Kit 121.00 €
CM4400 Human Aorta Smooth Muscle Cells (HAoSMC) Growth Supplements Complement with Human Muscle Cells Basal Medium 63.00 €
CM4401 Human Umbilical Vein Smooth Muscle Cells (HUVSMC) Growth Supplements Complement with Human Muscle Cells Basal Medium 63.00 €