BiobankCell BiologyBio-Analysis

Hepatocytes Media

Cost-effective and fully described media for human primary hepatocytes

CellMade hepatocytes cell media are using basal medium and functionally tested growth supplements for optimized growth of HEP. 

The basal media are MCDB-131 based. MCDB-131 medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HMVEC). MCDB-131 medium is also used for other cell types, including human hepatocytes and smooth muscle cells.

MCDB-131 medium contains many components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins. These additions allow the medium to be supplemented with very low levels of serum or defined components. MCDB-131 medium contains no proteins or growth factors, and has to be supplemented.

MCDB 131 Medium uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5 to 10% (V/V) CO2 environment to maintain physiological pH.

  • CM3500 contains Phenol Red
  • CM3501 does not contain Phenol Red
  • CM3500 and CM3501 do not contain HEPES
  • CM3500 and CM3501 do contain L-Glutamine

The complete formulation and description of the basal media and the different growth supplements are available under the Documentation Centre of this page.


Products

Ref Products Associated Products Price
CM3500 Human Hepatocytes Basal Medium, W/ Phenol Red, W/L-Glutamine, W/o HEPES, NAHCO3 buffered, 500 mL Complement with Cell Growth Supplements to obtain Growth Medium 58.00 €
CM3501 Human Hepatocytes Basal Medium, W/o Phenol Red, W/L-Glutamine, W/o HEPES, NAHCO3 buffered, 500 mL Complement with Cell Growth Supplements to obtain Growth Medium 58.00 €
CM5500 Human Hepatocytes Growth Medium, W/ Phenol Red, W/L-Glutamine, W/o HEPES, NAHCO3 buffered, and CM4500 growth supplements for HEP, 500 mL Complete Kit 121.00 €
CM5501 Human Hepatocytes Growth Medium, W/ Phenol Red, W/L-Glutamine, W/o HEPES, NAHCO3 buffered, and CM4500 growth supplements for HEP, 500 mL Complete Kit 121.00 €
CM4500 Human Hepatocytes (HEP) Growth Supplements Complement with Human Hepatocytes Basal Medium 63.00 €