Endothelial Cells Media
Cost-effective and fully described media for human primary endothelial cells
CellMade endothelial cell media are using basal medium and functionally tested growth supplements for optimized growth of HUVEC, HAoEC and HDMVEC.
The basal media are MCDB-131 based. MCDB-131 medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HMVEC). MCDB-131 medium is also used for other cell types, including human hepatocytes and smooth muscle cells.
MCDB-131 medium contains many components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins. These additions allow the medium to be supplemented with very low levels of serum or defined components. MCDB-131 medium contains no proteins or growth factors, and has to be supplemented.
MCDB 131 Medium uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5 to 10% (V/V) CO2 environment to maintain physiological pH.
- CM3100 contains Phenol Red
- CM3101 does not contain Phenol Red
- CM3100 and CM3101 do not contain HEPES
- CM3100 and CM3101 do contain L-Glutamine
The complete formulation and description of the basal media and the different growth supplements are available under the Documentation Centre of this page.
Documentation
Products
| Ref | Products | Associated Products | Price |
|---|---|---|---|
| CM3100 | Human Endothelial Cells Basal Medium, W/ Phenol Red, W/L-Glutamine, W/o HEPES, NAHCO3 buffered, 500 mL | Complement with Cell Growth Supplements to obtain Growth Medium | 58.00 € |
| CM3101 | Human Endothelial Cells Basal Medium, W/o Phenol Red, W/L-Glutamine, W/o HEPES, NAHCO3 buffered, 500 mL | Complement with Cell Growth Supplements to obtain Growth Medium | 58.00 € |
| CM5100 | Human Endothelial Cells Growth Medium W/Phenol Red, W/L-Glutamine, W/o HEPES, NAHCO3 buffered and CM-4100 growth supplements, for HDMVEC, 500 mL | Complete Kit | 121.00 € |
| CM5101 | Human Endothelial Cells Growth Medium, W/o Phenol Red, W/L-Glutamine, W/o HEPES, NAHCO3 buffered and CM-4100 growth supplements, for HDMVEC, 500 mL | Complete Kit | 121.00 € |
| CM5102 | Human Endothelial Cells Growth Medium W/Phenol Red, W/L-Glutamine, W/o HEPES, NAHCO3 buffered and CM-4101 growth supplements, for HAoEc, HUVEC, 500 mL | Complete Kit | 121.00 € |
| CM5103 | Human Endothelial Cells Growth Medium W/o Phenol Red, W/L-Glutamine, W/o HEPES, NAHCO3 buffered and CM-4101 growth supplements, for HAoEc, HUVEC, 500 mL | Complete Kit | 121.00 € |
| CM4100 | Human Dermal Microvascular Endothelial Cells (HDMVEC) Growth Supplements | Complement with Human Endothelial Cells Basal Medium | 63.00 € |
| CM4101 | Human Endothelial Cells (HUVEC, HAoEc) Growth Supplements | Complement with Human Endothelial Cells Basal Medium | 63.00 € |