Amino acids play a critical role in biochemistry from forming building blocks for proteins to acting as signaling molecules and lipid transport. The rapid quantification is essential to understand biochemistry of normal versus disease status. UPLC/MS/MS methods allow for rapid and simple quantification of amino acids.
Metabolic syndrome and its associated pathologies such as Obesity, Type II Diabetes, Cardiovascular Disease (e.g. Atherosclerosis, Stroke and Hypertension) have been related with circulating levels of several amino acids and metabolites. Plasma free amino acid (PFAA) profile can potentially evaluate individuals’ future risks of developing lifestyle-related diseases, in addition to diabetes. Indeed, it has been reported that the branched-chain amino acids/aromatic amino acids (BCAA/AAA) related pattern and the Glutamine/Glycine/Serine/Asparagine (Gln/Gly/Ser/Asn) related pattern were significantly associated with the Metabolic Syndrome. The BCAA/AAA pattern was positively associated, while the Gln/Gly/Ser/Asn pattern was inversely associated with established cardiometabolic risk factors and Metabolic Syndrome. It is interesting to underline that plasma Adiponectin levels or markers for liver function did not significantly affect these associations.
For further information on these assays and our standard experimental set-up, please download the CellMade Mass Spectrometry Assays – Technical Information and General Instructions leaflet.