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GMO - PCR services

Ensure EU Regulation 1829/2003 compliance with CellMade’s Real-Time PCR (qPCR) GMO testing. High-sensitivity detection (0.01% LOD) and 0.9% threshold quantification for processed food and feed.

In the global food and feed supply chain, the management of Genetically Modified Organisms (GMOs) is a critical regulatory pillar. Within the European Union, Regulations (EC) No 1829/2003 and 1830/2003 mandate the strict labeling of products containing more than 0.9% authorized GMOs.

Because aggressive industrial processing—such as high-heat extrusion, solvent extraction, or fermentation—often denatures proteins, DNA-based analysis via Real-Time PCR (qPCR) is the only definitive method for threshold compliance and identity preservation.

The Gold Standard for Regulatory Compliance

Real-time PCR targets specific, thermally stable DNA sequences (promoters, terminators, or event-specific junctions). This molecular approach provides the empirical evidence required for EFSA alignment and GFSI audit readiness.

  • Quantitative Precision: Allows for the exact quantification of the transgene relative to a species-specific reference gene, ensuring adherence to the EU 0.9% labeling threshold.
  • Exceptional Sensitivity: Achieves a Limit of Detection (LOD) down to 0.01%, essential for "Non-GMO" claim validation and Identity Preservation (IP) systems.
  • Matrix Resilience: Genomic DNA remains detectable in highly processed matrices (e.g., lecithin, corn starch, or refined oils) where protein-based ELISA tests often fail.

CellMade™ GMO PCR Analytical Suite

CellMade Laboratories provides a hierarchical qPCR framework designed to navigate the expanding landscape of global transgenic events.

1. Broad-Spectrum Screening

Efficiently monitor raw materials by targeting common regulatory elements found in the vast majority of commercial GMOs:

  • P-35S: Cauliflower Mosaic Virus promoter.
  • T-NOS: Agrobacterium tumefaciens terminator.
  • P-FMV: Figwort Mosaic Virus promoter.

2. Event-Specific Identification

When a screening result is positive, our event-specific assays identify the exact transgenic event (e.g., MON810, GTS 40-3-2) to determine if the GMO is authorized within the EU territory.

3. Stacked Trait Resolution

Advanced multiplex PCR capabilities allow for the simultaneous detection of multiple DNA targets in a single run, increasing laboratory throughput and resolving complex botanical impurities.

Streamlined Molecular Workflow

Our systems are engineered for high specificity and seamless integration into industrial quality control:

  • Internal Amplification Controls (IAC): Every service method includes EACs and IACs to eliminate the risk of false negatives caused by PCR inhibitors or system failures.
  • Universal Cycling Parameters: Streamlined protocols compatible with our Real-Time PCR instruments.
  • Clear Interpretation: Quantitative results are delivered via cycle threshold (Ct) analysis for absolute transparency.
  • Reports: available in 24-48h upon receipt of samples.